Es wurden qualitativ hochwertige Bilder erhalten, ein 3D-Modell erstellt und der Wirkungsmechanismus von nanoskaligen bakteriellen Biomotoren analysiert

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High-torque bacterial flagellar motors assemble large periplasmic disk complexes. (A–C) Tomographic slices through intact cells of Salmonella (A), V. fischeri (B), and C. jejuni © showing individual flagellar motors. Height of all image panels is 100 nm. (D–F) Slices (100 × 100 × 0.81 nm) through subtomogram averages of hundreds of motors. Color keys indicate the regions of the motor (named in G–I), of Salmonella (average of 286 motors) (D), V. fischeri (average of 302 motors) (E), and C. jejuni (average of 156 motors) (F). (G–I) Isosurface renderings of motors shown in D–F. FliI and FlhAC are components of the flagellar type III secretion system.

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Unsymmetrized motors showing clear symmetries. C. jejuni motors (A, Left) exhibited clear 17-fold symmetry (A, Right), which was applied to improve the signal-to-noise ratio (B). V. fischeri exhibited 13-fold symmetry ©, which was applied to improve the signal-to-noise ratio (D).

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newscientist.com
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Source: https://habr.com/ru/post/de391999/


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